A Viability Quantitative PCR Dilemma: Are Longer Amplicons Better?

The development of viability quantitative PCR (v-qPCR) has allowed for a more accurate assessment of the viability of a microbial sample by limiting the amplification of DNA from dead cells. Although valuable, v-qPCR is not infallible. One of the most limiting factors for accurate live/dead distinct...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Main Authors: Van Holm, Wannes, Ghesquière, Justien, Boon, Nico, Verspecht, Tim, Bernaerts, Kristel, Zayed, Naiera, Chatzigiannidou, Ioanna, Teughels, Wim
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2021
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8090887/
https://ncbi.nlm.nih.gov/pubmed/33361365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02653-20
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