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Highly efficient and safe genome editing by CRISPR-Cas12a using CRISPR RNA with a ribosyl-2′-O-methylated uridinylate-rich 3′-overhang in mouse zygotes

The CRISPR-Cas12a system has been developed to harness highly specific genome editing in eukaryotic cells. Given the relatively small sizes of Cas12a genes, the system has been suggested to be most applicable to gene therapy using AAV vector delivery. Previously, we reported that a U-rich crRNA enab...

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Dettagli Bibliografici
Pubblicato in:Exp Mol Med
Autori principali: Ha, Dae-In, Lee, Jeong Mi, Lee, Nan-Ee, Kim, Daesik, Ko, Jeong-Heon, Kim, Yong-Sam
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8080787/
https://ncbi.nlm.nih.gov/pubmed/33162553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s12276-020-00521-7
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