Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortex

Here, we present a comprehensive protocol to analyze the roles of disease-related genes in synaptic transmission. We have developed a pipeline of electrophysiological techniques and combined these with optogenetics in the medial prefrontal cortex of mice. This methodology provides a cost-effective,...

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Sparad:
Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Nagahama, Kenichiro, Fujino, Shuhei, Watanabe, Takaki, Uesaka, Naofumi, Kano, Masanobu
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2021
Ämnen:
Protocol
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC8079664/
https://ncbi.nlm.nih.gov/pubmed/33937875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100469
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