Messenger RNA capture sequencing of extracellular RNA from human biofluids using a comprehensive set of spike-in controls

Comprehensive transcriptome analysis of extracellular RNA (exRNA) purified from human biofluids is challenging because of the low RNA concentration and compromised RNA integrity. Here, we describe an optimized workflow to (1) isolate exRNA from different types of biofluids and (2) to prepare messeng...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Hulstaert, Eva, Decock, Anneleen, Morlion, Annelien, Everaert, Celine, Verniers, Kimberly, Nuytens, Justine, Nijs, Nele, Schroth, Gary P., Kuersten, Scott, Gross, Stephen M., Mestdagh, Pieter, Vandesompele, Jo
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8076706/
https://ncbi.nlm.nih.gov/pubmed/33937877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100475
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