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Primary mouse osteoblast and osteoclast culturing and analysis

Mesenchymal-derived osteoblasts play a key role in bone formation via synthesis and mineralization of the bone and bone remodeling. Osteoclasts are multinucleated cells of hematopoietic origin with a role in bone resorption. Here, we describe a protocol for generating primary cultures of these two c...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Chevalier, Claire, Çolakoğlu, Melis, Brun, Julia, Thouverey, Cyril, Bonnet, Nicolas, Ferrari, Serge, Trajkovski, Mirko
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8065294/
https://ncbi.nlm.nih.gov/pubmed/33912848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100452
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