CRISPR-based DNA and RNA detection with liquid-liquid phase separation

The ability to detect specific nucleic acid sequences allows for a wide range of applications such as the identification of pathogens, clinical diagnostics, and genotyping. CRISPR-Cas proteins Cas12a and Cas13a are RNA-guided endonucleases that bind and cleave specific DNA and RNA sequences, respect...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Spoelstra, Willem Kasper, Jacques, Jeroen M., Gonzalez-Linares, Rodrigo, Nobrega, Franklin L., Haagsma, Anna C., Dogterom, Marileen, Meijer, Dimphna H., Idema, Timon, Brouns, Stan J.J., Reese, Louis
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2021
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8059199/
https://ncbi.nlm.nih.gov/pubmed/33617832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2021.02.013
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