4-O-Methyl Modifications of Glucuronic Acids in Xylans Are Indispensable for Substrate Discrimination by GH67 α-Glucuronidase from Bacillus halodurans C-125

A GH67 α-glucuronidase gene derived from Bacillus halodurans C-125 was expressed in E. coli to obtain a recombinant enzyme (BhGlcA67). Using the purified enzyme, the enzymatic properties and substrate specificities of the enzyme were investigated. BhGlcA67 showed maximum activity at pH 5.4 and 45 °C...

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Detalhes bibliográficos
Publicado no:J Appl Glycosci (1999)
Main Authors: Yagi, Haruka, Maehara, Tomoko, Tanaka, Tsuyoshi, Takehara, Ryo, Teramoto, Koji, Yaoi, Katsuro, Kaneko, Satoshi
Formato: Artigo
Idioma:Inglês
Publicado em: The Japanese Society of Applied Glycoscience 2017
Assuntos:
Regular Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8056927/
https://ncbi.nlm.nih.gov/pubmed/34354504
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5458/jag.jag.JAG-2017_016
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