Acidic pH Decreases the Endonuclease Activity of Initiator RepB and Increases the Stability of the Covalent RepB-DNA Intermediate while Has Only a Limited Effect on the Replication of Plasmid pMV158 in Lactococcus lactis

Plasmid vectors constitute a valuable tool for homologous and heterologous gene expression, for characterization of promoter and regulatory regions, and for genetic manipulation and labeling of bacteria. During the last years, a series of vectors based on promiscuous replicons of the pMV158 family h...

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Bibliographic Details
Published in:Front Mol Biosci
Main Authors: Valdelvira, Rafael, Bordanaba-Ruiseco, Lorena, Martín-Huestamendía, Cristina, Ruiz-Masó, José Angel, del Solar, Gloria
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2021
Subjects:
Molecular Biosciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8056398/
https://ncbi.nlm.nih.gov/pubmed/33889596
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmolb.2021.634461
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