Protocol for live enhanced resolution confocal imaging of dendritic spinule dynamics in primary mouse cortical neuron culture

Dendritic spinules are fine membranous protrusions of neuronal spines that play a role in synaptic plasticity, but their nanoscale requires resolution beyond conventional confocal microscopy, hindering live studies. Here, we describe how to track individual spinules in live dissociated cortical pyra...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Zaccard, Colleen R., Kirchenbuechler, David, Yoon, Sehyoun, Arvanitis, Constadina, Penzes, Peter
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8056271/
https://ncbi.nlm.nih.gov/pubmed/33899014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100427
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