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Comparison of Free Zinc Levels Determined by Fluorescent Probes in THP1 Cells Using Microplate Reader and Flow Cytometer

Free zinc is involved in signal transduction within mammalian cells, acting as a second messenger. Gold standard for its analysis is currently the use of metal-responsive fluorescent probes. The present study elucidates the impact of instrumentation used for measuring the resulting fluorescence. The...

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Detalhes bibliográficos
Publicado no:Biol Trace Elem Res
Main Authors: Alker, Wiebke, Haase, Hajo
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8055576/
https://ncbi.nlm.nih.gov/pubmed/32865725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12011-020-02355-w
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