Solid-phase XRN1 reactions for RNA cleavage: application in single-molecule sequencing

Modifications in RNA are numerous (∼170) and in higher numbers compared to DNA (∼5) making the ability to sequence an RNA molecule to identify these modifications highly tenuous using next generation sequencing (NGS). The ability to immobilize an exoribonuclease enzyme, such as XRN1, to a solid supp...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Athapattu, Uditha S, Amarasekara, Charuni A, Immel, Jacob R, Bloom, Steven, Barany, Francis, Nagel, Aaron C, Soper, Steven A
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2021
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8053086/
https://ncbi.nlm.nih.gov/pubmed/33511416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab001
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