Surface-Templated Nanobubbles Protect Proteins from Surface-Mediated Denaturation

In this paper, we report that surface-bound nanobubbles reduce protein denaturation on methylated glass by irreversible protein shell formation. Single-molecule total internal reflection fluorescence (SM-TIRF) microscopy was combined with intramolecular Förster Resonance Energy Transfer (FRET) to st...

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Publicado en:J Phys Chem Lett
Main Authors: Bull, David S., Kienle, Daniel F., Chaparro Sosa, Andres F., Nelson, Nathaniel, Roy, Shambojit, Cha, Jennifer N., Schwartz, Daniel K., Kaar, Joel L., Goodwin, Andrew P.
Formato: Artigo
Idioma:Inglês
Publicado: 2019
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8051143/
https://ncbi.nlm.nih.gov/pubmed/31067058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpclett.9b00806
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