Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins in Caenorhabditis elegans

We create and share a new red fluorophore, along with a set of strains, reagents and protocols, to make it faster and easier to label endogenous Caenorhabditis elegans proteins with fluorescent tags. CRISPR-mediated fluorescent labeling of C. elegans proteins is an invaluable tool, but it is much mo...

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Bibliografiske detaljer
Udgivet i:Genetics
Main Authors: Goudeau, Jérôme, Sharp, Catherine S, Paw, Jonathan, Savy, Laura, Leonetti, Manuel D, York, Andrew G, Updike, Dustin L, Kenyon, Cynthia, Ingaramo, Maria
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2021
Fag:
Investigation
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8049552/
https://ncbi.nlm.nih.gov/pubmed/33693628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/genetics/iyab014
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