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Separation of the cell envelope for Gram-negative bacteria into inner and outer membrane fractions with technical adjustments for Acinetobacter baumannii.
This method works by partitioning the envelope of Gram-negative bacteria into total, inner, and outer-membrane (OM) fractions and concludes with assays to assess the purity of the bilayers. The OM has an increased overall density compared to the inner membrane, largely due to the presence of lipooli...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8045671/ https://ncbi.nlm.nih.gov/pubmed/32338649 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60517 |
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