Modified PCR protocol to increase sensitivity for determination of bacterial community composition

BACKGROUND: The objective of this project was to increase the sensitivity of sequence-based bacterial community determination without impacting community composition or interfering with cluster formation during sequencing. Two PCR protocols (standard and modified) were examined in airway samples whe...

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Bibliografiske detaljer
Udgivet i:Microbiome
Main Authors: Williamson, Kayla M., Wagner, Brandie D., Robertson, Charles E., Stevens, Mark J., Sontag, Marci K., Mourani, Peter M., Harris, J. Kirk
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2021
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8045227/
https://ncbi.nlm.nih.gov/pubmed/33849648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40168-020-00958-y
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