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Protocol to produce high-titer retrovirus for transduction of mouse bone marrow cells

Regulating gene expression through retroviral infection has been widely used in mouse bone marrow transplantation (BMT) to test the capacity of self-renewal, as well as multi-lineage differentiation of hematopoietic stem and progenitor cells (HSPCs). However, it remains challenging to achieve high t...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Principais autores: Qu, Jing, Yang, Zhenhua
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8038942/
https://ncbi.nlm.nih.gov/pubmed/33851142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100459
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