An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2

Human angiotensin converting enzyme 2 (hACE2) mediates the cell entry of both SARS-CoV and SARS-CoV2 and can be used as a drug target. The DNA encoding the truncated hACE2 (30-356aa) was cloned into pET-28a (+) and expressed in Escherichia coli Rosetta (DE3). The recombinant hACE2 (rhACE2) was purif...

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Библиографические подробности
Опубликовано в: :Protein Expr Purif
Главные авторы: Gao, Xiangzheng, Liang, Keying, Mei, Shengsheng, Peng, Shanshan, Vong, Eu Gene, Zhan, Jinbiao
Формат: Artigo
Язык:Inglês
Опубликовано: Elsevier Inc. 2021
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Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC8036248/
https://ncbi.nlm.nih.gov/pubmed/33852951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2021.105889
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