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Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization

Human norovirus (NoV) is the leading cause of acute viral gastroenteritis and a major source of foodborne illness. Detection of NoV in food and environmental samples is typically performed using molecular techniques, including real-time reverse transcription polymerase chain reaction (RT-PCR) and le...

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Publicat a:PLoS One
Autors principals: Manuel, Clyde S., Suther, Cassandra, Moore, Matthew D., Jaykus, Lee-Ann
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8031402/
https://ncbi.nlm.nih.gov/pubmed/33831019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0248581
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