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Protocol for fractionation-assisted native ChIP (fanChIP) to capture protein-protein/DNA interactions on chromatin

Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/chromatin complexes applicable for analyses of both pr...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Miyamoto, Ryo, Yokoyama, Akihiko
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8024768/
https://ncbi.nlm.nih.gov/pubmed/33855306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100404
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