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Analysis of cardiac chamber development during mouse embryogenesis using whole mount epifluorescence

The goal of this protocol is to describe a method for dissection of mouse embryos and visualization of embryonic mouse ventricular chambers during heart development using ventricular specific fluorescent reporter knock-in mice (MLC-2v-tdTomato mice). Heart development involves a linear heart tube fo...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Zhang, Zhentao, Nam, Young-Jae
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8017474/
https://ncbi.nlm.nih.gov/pubmed/31058904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59413
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