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Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

Profound transcriptional heterogeneity in anatomically adjacent single-cells suggests robust tissue functionality may be achieved by cellular phenotype diversity. Single-cell experiments investigating the network dynamics of biological systems demonstrate cellular and tissue responses to various con...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: O’Sullivan, Sean J., Reyes, Beverly A.S., Vadigepalli, Rajanikanth, Van Bockstaele, Elisabeth J., Schwaber, James S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8015684/
https://ncbi.nlm.nih.gov/pubmed/32202523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60612
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