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Quantitative analysis of RNA chaperone activity by native gel electrophoresis and fluorescence spectroscopy

Diverse types of RNA binding proteins chaperone the interactions of non-coding RNAs by increasing the rate of RNA base pairing and by stabilizing the final RNA duplex. The E. coli protein Hfq facilitates interactions between small non-coding RNAs and their target mRNAs. The chaperone and RNA anneali...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Panja, Subrata, Małecka, Ewelina M., Santiago-Frangos, Andrew, Woodson, Sarah A.
Format: Artigo
Sprog:Inglês
Udgivet: 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8015265/
https://ncbi.nlm.nih.gov/pubmed/31889249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0231-7_2
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