Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice

We transduced mouse cortical astrocytes cultured from four litters of embryonic wildtype (WT) and connexin43 (Cx43) null mouse pups with lentiviral vector encoding hTERT and measured expression of astrocyte-specific markers up to passage 10 (p10). The immortalized cell lines thus generated (designat...

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Publicat a:Front Cell Neurosci
Autors principals: Cibelli, Antonio, Veronica Lopez-Quintero, Sandra, Mccutcheon, Sean, Scemes, Eliana, Spray, David C., Stout, Randy F., Suadicani, Sylvia O., Thi, Mia M., Urban-Maldonado, Marcia
Format: Artigo
Idioma:Inglês
Publicat: Frontiers Media S.A. 2021
Matèries:
Neuroscience
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8005635/
https://ncbi.nlm.nih.gov/pubmed/33790744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fncel.2021.647109
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