Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap

Next-generation sequencing has revolutionized our ability to investigate the microbiota composition of diverse and complex environments. However, a number of factors can affect the accuracy of microbial community assessment, such as the DNA extraction method, the hypervariable region of 16S rRNA gen...

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Bibliographic Details
Published in:Front Plant Sci
Main Authors: Haro, Carmen, Anguita-Maeso, Manuel, Metsis, Madis, Navas-Cortés, Juan A., Landa, Blanca B.
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2021
Subjects:
Plant Science
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7994608/
https://ncbi.nlm.nih.gov/pubmed/33777075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2021.640829
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