Protocol for intervention-free quantification of protein turnover rate by steady-state modeling

Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This approach can be used to calculate production and d...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Marcotti, Stefania, Sánchez-Sánchez, Besaiz Jose, Serna-Morales, Eduardo, Dragu, Anca, Díaz-de-la-Loza, María-del-Carmen, Matsubayashi, Yutaka, Stramer, Brian Marc
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7988239/
https://ncbi.nlm.nih.gov/pubmed/33786460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100377
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