Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as b...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Bojmar, Linda, Kim, Han Sang, Tobias, Gabriel C., Pelissier Vatter, Fanny A., Lucotti, Serena, Gyan, Kofi Ennu, Kenific, Candia M., Wan, Zurong, Kim, Kyung-A, Kim, DooA, Hernandez, Jonathan, Pascual, Virginia, Heaton, Todd E., La Quaglia, Michael P., Kelsen, David, Trippett, Tanya M., Jones, David R., Jarnagin, William R., Matei, Irina R., Zhang, Haiying, Hoshino, Ayuko, Lyden, David
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7988237/
https://ncbi.nlm.nih.gov/pubmed/33786456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100225
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