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An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple...

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Publicat a:STAR Protoc
Autors principals: Lyra-Leite, Davi Marco, Fonoudi, Hananeh, Gharib, Mennat, Burridge, Paul W.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7988236/
https://ncbi.nlm.nih.gov/pubmed/33786455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100213
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