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Functional proteomics protocol for the identification of interaction partners in Tetrahymena thermophila
We describe an optimized protocol for one-step affinity purification of FZZ-tagged proteins followed by mass spectrometry analysis for the identification of protein-protein interactions in the ciliate protozoan Tetrahymena thermophila. The FZZ epitope tag contains 2 protein A moieties (ZZ) and a 3xF...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7988224/ https://ncbi.nlm.nih.gov/pubmed/33786459 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100362 |
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