Functional proteomics protocol for the identification of interaction partners in Tetrahymena thermophila

We describe an optimized protocol for one-step affinity purification of FZZ-tagged proteins followed by mass spectrometry analysis for the identification of protein-protein interactions in the ciliate protozoan Tetrahymena thermophila. The FZZ epitope tag contains 2 protein A moieties (ZZ) and a 3xF...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Nabeel-Shah, Syed, Garg, Jyoti, Kougnassoukou Tchara, Pata-Eting, Pearlman, Ronald E., Lambert, Jean-Philippe, Fillingham, Jeffrey
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7988224/
https://ncbi.nlm.nih.gov/pubmed/33786459
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100362
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