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Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond network

A high degree of charge heterogeneity is an unfavorable phenomenon commonly observed for therapeutic monoclonal antibodies (mAbs). Removal of these impurities during manufacturing often comes at the cost of impaired step yields. A wide spectrum of posttranslational and chemical modifications is know...

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Vydáno v:Biotechnol Bioeng
Hlavní autoři: Prade, Elke, Zeck, Anne, Stiefel, Fabian, Unsoeld, Andreas, Mentrup, David, Arango Gutierrez, Erik, Gorr, Ingo H.
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7986432/
https://ncbi.nlm.nih.gov/pubmed/33200817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.27628
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