Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy

Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging s...

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Publicado en:J Struct Biol X
Main Authors: Lane, Ryan, Vos, Yoram, Wolters, Anouk H.G., Kessel, Luc van, Chen, S. Elisa, Liv, Nalan, Klumperman, Judith, Giepmans, Ben N.G., Hoogenboom, Jacob P.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7973379/
https://ncbi.nlm.nih.gov/pubmed/33763642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.yjsbx.2021.100046
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