Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification

High-throughput screening methodologies to estimate lipid content in oleaginous yeasts use Nile red fluorescence in a given solvent and optimized excitation/emission wavelengths. However, Nile red fluorescence stabilization has been poorly analyzed, and high variability occurs when relative fluoresc...

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發表在:Front Microbiol
Main Authors: Ramírez-Castrillón, Mauricio, Jaramillo-Garcia, Victoria P., Lopes Barros, Helio, Pegas Henriques, João A., Stefani, Valter, Valente, Patricia
格式: Artigo
語言:Inglês
出版: Frontiers Media S.A. 2021
主題:
Microbiology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7969498/
https://ncbi.nlm.nih.gov/pubmed/33746916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2021.619313
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