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Analysis and uncertainty quantification of DNA fluorescence melt data: Applications of affine transformations

Fluorescence-based measurements are a standard tool for characterizing the thermodynamic properties of DNA systems. Nonetheless, experimental melt data obtained from polymerase chain-reaction (PCR) machines (for example) often leads to signals that vary significantly between datasets. In many cases,...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Patrone, Paul N., Kearsley, Anthony J., Majikes, Jacob M., Liddle, J. Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7961812/
https://ncbi.nlm.nih.gov/pubmed/32526200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2020.113773
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