Purification of mouse hepatic non-parenchymal cells or nuclei for use in ChIP-seq and other next-generation sequencing approaches

Significant advancements in understanding disease mechanisms can occur through combined analysis of next-generation sequencing datasets generated using purified cell populations. Here, we detail our optimized protocol for purification of mouse hepatic macrophages (or other liver non-parenchymal popu...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Troutman, Ty D., Bennett, Hunter, Sakai, Mashito, Seidman, Jason S., Heinz, Sven, Glass, Christopher K.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7960533/
https://ncbi.nlm.nih.gov/pubmed/33748781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100363
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