Carboxyfluorescein Dye Uptake to Measure Connexin-mediated Hemichannel Activity in Cultured Cells

Connexins are membrane bound proteins that facilitate direct and local paracrine mediated cell-to-cell communication through their ability to oligomerise into hexameric hemichannels. When neighbouring channels align, they form gap-junctions that provide a direct route for information transfer betwee...

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Udgivet i:Bio Protoc
Main Authors: Potter, Joe A., Price, Gareth W., Cliff, Chelsy L., Williams, Bethany M., Hills, Claire E., Squires, Paul E.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2021
Fag:
Methods Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7952939/
https://ncbi.nlm.nih.gov/pubmed/33732788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3901
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