Purification of Cytosolic Phospholipase A(2)α C2-domain after Expression in Soluble Form in Escherichia coli

Previous expression/purification strategies for cytosolic phospholipase A(2)α C2-domain in Escherichia coli have relied on refolded protein recovered from inclusion bodies and sometimes containing C-terminal Cys139Ala and Cys141Ser substitutions to eliminate potential refolding complications induced...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Hirano, Yoshinori, Gao, Yong-Guang, K. Simanshu, Dhirendra, J. Stephenson, Daniel, T. Vu, Ngoc, Malinina, Lucy, E. Chalfant, Charles, J. Patel, Dinshaw, E. Brown, Rhoderick
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2021
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Methods Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7952920/
https://ncbi.nlm.nih.gov/pubmed/33732793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3906
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