Analysis of reactive astrogliosis in mouse brain using in situ hybridization combined with immunohistochemistry

Reactive astrogliosis is characterized by a profound change in astrocyte phenotype in response to all CNS injuries. Here, we present a revised in situ hybridization and immunohistochemistry (IHC) protocol to label the reactive astrocytes in the mouse brain. Several approaches for quantifying astrocy...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:STAR Protoc
Autori principali: Muraleedharan, Ranjithmenon, Nardini, Diana, Waclaw, Ronald Raymond, Dasgupta, Biplab
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2021
Soggetti:
Protocol
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7933799/
https://ncbi.nlm.nih.gov/pubmed/33718893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100375
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