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Miniaturized filter-aided sample preparation (MICRO-FASP) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus laevis embryos
We report a miniaturized Filter Aided Sample Preparation method (micro-FASP) for low-loss preparation of submicrogram proteomic samples. The method employs a filter with ~0.1 mm(2) surface area, reduces the total volume of reagents to < 10 μL, and requires only two sample transfer steps. The meth...
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| Published in: | Anal Chem |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7931810/ https://ncbi.nlm.nih.gov/pubmed/32125139 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c00470 |
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