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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Proximity labeling (PL) techniques using engineered ascorbate peroxidase (APEX) or Escherichia coli biotin ligase BirA (known as BioID) have been successfully used for identification of protein-protein interactions (PPIs) in mammalian cells. However, requirements of toxic hydrogen peroxide (H(2)O(2)...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7927212/ https://ncbi.nlm.nih.gov/pubmed/32478742 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60728 |
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