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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Proximity labeling (PL) techniques using engineered ascorbate peroxidase (APEX) or Escherichia coli biotin ligase BirA (known as BioID) have been successfully used for identification of protein-protein interactions (PPIs) in mammalian cells. However, requirements of toxic hydrogen peroxide (H(2)O(2)...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Zhang, Yongliang, Li, Yuanyuan, Yang, Xinxin, Wen, Zhiyan, Nagalakshmi, Ugrappa, Dinesh-Kumar, Savithramma P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7927212/
https://ncbi.nlm.nih.gov/pubmed/32478742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60728
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