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An optimized protocol for rapid, sensitive and robust on-bead ChIP-seq from primary cells

Integrative analysis of next-generation sequencing data can help understand disease mechanisms. Specifically, ChIP-seq can illuminate where transcription regulators bind to regulate transcription. A major obstacle to performing this assay on primary cells is the low numbers obtained from tissues. Th...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Texari, Lorane, Spann, Nathanael J., Troutman, Ty D., Sakai, Mashito, Seidman, Jason S., Heinz, Sven
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7921621/
https://ncbi.nlm.nih.gov/pubmed/33718886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100358
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