Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics

Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible for rare cell p...

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Udgivet i:Commun Biol
Main Authors: Tsai, Chia-Feng, Zhang, Pengfei, Scholten, David, Martin, Kendall, Wang, Yi-Ting, Zhao, Rui, Chrisler, William B., Patel, Dhwani B., Dou, Maowei, Jia, Yuzhi, Reduzzi, Carolina, Liu, Xia, Moore, Ronald J., Burnum-Johnson, Kristin E., Lin, Miao-Hsia, Hsu, Chuan-Chih, Jacobs, Jon M., Kagan, Jacob, Srivastava, Sudhir, Rodland, Karin D., Steven Wiley, H., Qian, Wei-Jun, Smith, Richard D., Zhu, Ying, Cristofanilli, Massimo, Liu, Tao, Liu, Huiping, Shi, Tujin
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7921383/
https://ncbi.nlm.nih.gov/pubmed/33649493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-021-01797-9
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