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Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer
Epitope tags are widely employed as tools to detect, purify and manipulate proteins in various experimental systems. We recently introduced the ALFA-tag together with two ALFA-specific single-domain antibodies (sdAbs), NbALFA and NbALFA(PE), featuring high or intermediate affinity, respectively. Tog...
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| Publicado no: | Biomolecules |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MDPI
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7918568/ https://ncbi.nlm.nih.gov/pubmed/33673130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/biom11020269 |
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