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Long-Range PCR-Based NGS Applications to Diagnose Mendelian Retinal Diseases

The purpose of this study was to develop a flexible, cost-efficient, next-generation sequencing (NGS) protocol for genetic testing. Long-range polymerase chain reaction (PCR) amplicons of up to 20 kb in size were designed to amplify entire genomic regions for a panel (n = 35) of inherited retinal di...

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Dades bibliogràfiques
Publicat a:Int J Mol Sci
Autors principals: Maggi, Jordi, Koller, Samuel, Bähr, Luzy, Feil, Silke, Kivrak Pfiffner, Fatma, Hanson, James V. M., Maspoli, Alessandro, Gerth-Kahlert, Christina, Berger, Wolfgang
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7913364/
https://ncbi.nlm.nih.gov/pubmed/33546218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22041508
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