Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA

The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The b...

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Veröffentlicht in:Microorganisms
Hauptverfasser: Petrovskis, Ivars, Lieknina, Ilva, Dislers, Andris, Jansons, Juris, Bogans, Janis, Akopjana, Inara, Zakova, Jelena, Sominskaya, Irina
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2021
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7912224/
https://ncbi.nlm.nih.gov/pubmed/33573151
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/microorganisms9020283
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