Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues

Advanced-stage gynecologic cancer remains a life-threatening disease. Here, we present a protocol for establishment of stable in vitro 3D spheroid cells derived from human uterine endometrial and ovarian cancer tissues. The tumor-derived spheroid cells have cancer stem cell-related characteristics,...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Ueda, Haruka, Mori, Yutaro, Yamawaki, Kaoru, Ishiguro, Tatsuya, Ohata, Hirokazu, Sato, Ai, Sugino, Kentaro, Yachida, Nozomi, Yamaguchi, Manako, Suda, Kazuaki, Tamura, Ryo, Yoshihara, Kosuke, Okamoto, Koji, Enomoto, Takayuki
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7905465/
https://ncbi.nlm.nih.gov/pubmed/33665634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100354
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