Construction, characterization and crystal structure of a fluorescent single-chain Fv chimera

In vitro display technologies based on phage and yeast have a successful history of selecting single-chain variable fragment (scFv) antibodies against various targets. However, single-chain antibodies are often unstable and poorly expressed in Escherichia coli. Here, we explore the feasibility of co...

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Detalhes bibliográficos
Publicado no:Protein Eng Des Sel
Main Authors: Velappan, Nileena, Close, Devin, Hung, Li-Wei, Naranjo, Leslie, Hemez, Colin, DeVore, Natasha, McCullough, Donna K, Lillo, Antonietta M, Waldo, Geoffrey S, Bradbury, Andrew R M
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2021
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7901706/
https://ncbi.nlm.nih.gov/pubmed/33586761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzaa029
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