In Situ Labeling and Distance Measurements of Membrane Proteins in E. coli Using Finland and OX063 Trityl Labels

In situ investigation of membrane proteins is a challenging task. Previously we demonstrated that nitroxide labels combined with pulsed ESR spectroscopy is a promising tool for this purpose. However, the nitroxide labels suffer from poor stability, high background labeling, and low sensitivity. Here...

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Bibliographic Details
Published in:Chemistry
Main Authors: Ketter, Sophie, Gopinath, Aathira, Rogozhnikova, Olga, Trukhin, Dmitrii, Tormyshev, Victor M., Bagryanskaya, Elena G., Joseph, Benesh
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2021
Subjects:
Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7898545/
https://ncbi.nlm.nih.gov/pubmed/33197077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202004606
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