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A Novel Utility to Correct for Plate/Batch/Lot and Nonspecific Binding Artifacts in Luminex Data

Cytokines and other secreted soluble proteins are routinely assayed as fluorescence intensities on the Luminex (Luminex, Austin, TX) platform. As with any immunoassay, a portion of the measured Ab binding can be nonspecific. Use of spiked-in microbead controls (e.g., AssayChex Process, Control Panel...

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Detalhes bibliográficos
Publicado no:J Immunol
Main Authors: Maecker, Holden T., Rosenberg-Hasson, Yael, Kolstad, Kathleen Durgin, Steen, Virginia D., Chung, Lorinda S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7891557/
https://ncbi.nlm.nih.gov/pubmed/32376648
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.2000017
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