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CRISPR Turbo Accelerated KnockOut (CRISPy TAKO) for Rapid in vivo Screening of Gene Function

The development of CRISPR/Cas9 technology has vastly sped up the process of mammalian genome editing by introducing a bacterial system that can be exploited for reverse genetics-based research. However, generating homozygous functional knockout (KO) animals using traditional CRISPR/Cas9-mediated tec...

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Bibliographische Detailangaben
Veröffentlicht in:Front Genome Ed
Hauptverfasser: Plasil, Sonja L., Seth, Amit, Homanics, Gregg E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7889042/
https://ncbi.nlm.nih.gov/pubmed/33604589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fgeed.2020.598522
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