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Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes

BACKGROUND: DNA mismatches can affect the efficiency of PCR techniques if the intended target has mismatches in primer or probe regions. The accepted rule is that mismatches are detrimental as they reduce the hybridization temperatures, yet a more quantitative assessment is rarely performed. METHODS...

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Detalhes bibliográficos
Publicado no:Mol Cell Probes
Main Authors: Miranda, Pâmella, Weber, Gerald
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Ltd. 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7888997/
https://ncbi.nlm.nih.gov/pubmed/33609730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2021.101707
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