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Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes

BACKGROUND: DNA mismatches can affect the efficiency of PCR techniques if the intended target has mismatches in primer or probe regions. The accepted rule is that mismatches are detrimental as they reduce the hybridization temperatures, yet a more quantitative assessment is rarely performed. METHODS...

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書目詳細資料
發表在:Mol Cell Probes
Main Authors: Miranda, Pâmella, Weber, Gerald
格式: Artigo
語言:Inglês
出版: Elsevier Ltd. 2021
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7888997/
https://ncbi.nlm.nih.gov/pubmed/33609730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2021.101707
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