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Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes

BACKGROUND: DNA mismatches can affect the efficiency of PCR techniques if the intended target has mismatches in primer or probe regions. The accepted rule is that mismatches are detrimental as they reduce the hybridization temperatures, yet a more quantitative assessment is rarely performed. METHODS...

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Bibliografske podrobnosti
izdano v:Mol Cell Probes
Main Authors: Miranda, Pâmella, Weber, Gerald
Format: Artigo
Jezik:Inglês
Izdano: Elsevier Ltd. 2021
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7888997/
https://ncbi.nlm.nih.gov/pubmed/33609730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2021.101707
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