Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling

This protocol allows repeated whole-cell patch-clamp recordings from individual rodent CA1 hippocampal neurons, followed by immunohistological labeling of the axon initial segment. This overcomes the need to maintain whole-cell recordings over the timescales required for homeostatic modification to...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Main Authors: Oliveira, Laura S., Sumera, Anna, Booker, Sam A.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2021
Assuntos:
Protocol
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7887436/
https://ncbi.nlm.nih.gov/pubmed/33644771
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100336
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